Merck
CN
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文件

安全信息

14571C

SAFC

EX-CELL® 293无血清培养基，用于HEK 293细胞

Serum-free, Animal-protein free, without L-glutamine, liquid, suitable for cell culture

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MDL编号:
MFCD01634638
NACRES:
NA.75

无菌性

sterile; sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

组分

NaHCO3: yes
HEPES: no
L-glutamine: no
glucose: yes

运输

ambient

储存温度

2-8°C

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相关类别

应用

EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.
EX-CELL 293是一种不含动物蛋白的无血清培养基，用于人胚肾293（HEK 293）及相关细胞的长期生长。悬浮培养的细胞可以从补充血清培养基中直接传代培养到EX-CELL 293中，无需或仅需极少的适应期。悬浮培养物可以维持培养10天而无需换液，可传代20次以上而不损失存活率。

法律信息

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
动植物来源培养基

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Jan Bláha et al.
Protein expression and purification, 109, 7-13 (2015-01-28)
Lectin-like transcript 1 (LLT1, gene clec2d) was identified to be a ligand for the single human NKR-P1 receptor present on NK and NK-T lymphocytes. Naturally, LLT1 is expressed on the surface of NK cells, stimulating IFN-γ production, and is up-regulated
Jan Bláha et al.
Protein expression and purification, 140, 36-43 (2017-08-02)
Human natural killer receptor protein 1 (NKR-P1, CD161, gene klrb1) is a C-type lectin-like receptor of natural killer (NK) cells responsible for recognition of its cognate protein ligand lectin-like transcript 1 (LLT1). NKR-P1 is the single human orthologue of the
G Schoofs et al.
Cytotechnology, 28(1-3), 81-89 (2008-11-13)
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum
Pratik Jaluria et al.
Biotechnology and bioengineering, 99(6), 1443-1452 (2007-11-21)
Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of
J B Kaufman et al.
Cytotechnology, 33(1-3), 3-11 (2008-11-13)
The extracellular domain of human parathyroid Ca(2+) receptor was needed in order to study itsstructure and clinical application. The Ca(2+)receptor is a unique member of the G protein-coupledreceptor super-family, expressed in parathyroid andkidney cells where it has been shown to

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