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Merck
CN

C7559

氯仿

≥99%, PCR Reagent, contains amylenes as stabilizer

别名:

三氯甲烷

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关于此项目

经验公式(希尔记法):
CHCl3
化学文摘社编号:
分子量:
119.38
UNSPSC Code:
12191502
NACRES:
NA.28
PubChem Substance ID:
EC Number:
200-663-8
Beilstein/REAXYS Number:
1731042
MDL number:
Assay:
≥99%
Technique(s):
PCR: suitable
Bp:
60.5-61.5 °C (lit.)
Vapor pressure:
160 mmHg ( 20 °C)
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vapor density

4.1 (vs air)

Quality Level

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

form

liquid

contains

amylenes as stabilizer

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, none detected

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

General description

氯仿在药物制剂中可用作麻醉剂和添加剂。现在它主要用作有机溶剂。氯仿由人为和天然来源产生。这种在大气中含量丰富的卤化碳存在于地下水和地表水源中。

Application

氯仿已用于苯酚-氯仿提取,以考察其从血液培养液中纯化核酸的能力。它还用于 RNA 提取/分离和纯化。


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ppe

Eyeshields, Faceshields, Gloves

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

存储类别

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

危险化学品
易制毒化学品(2类)

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.