usage
sufficient for 50 purifications
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Aligned
General description
Norgen Biotek植物/真菌总RNA纯化试剂盒可以快速地从多种植物和丝状真菌种样本中分离和纯化出总RNA（包括病毒RNA）。通过该试剂盒，可从新鲜或冰冻的植物组织、植物细胞或丝状真菌样本之中纯化出总RNA。大到mRNA和rRNA，小到miRNA和siRNA，所有大小的RNA均可通过该试剂盒进行纯化。 该试剂盒不需要使用液氮来均质化样本，操作步骤快速、方便。
Application
用于从植物和丝状真菌中快速纯化总RNA（包括microRNA）。
纯化所得的RNA具有最高的质量，可用于诸多下游应用，包括：
- qRT-PCR
- 反转录PCR
- Northern杂交
- RNAse保护
- 引物延伸
- 表达阵列分析
- 二代测序
Features and Benefits
- 可从广泛的植物和丝状真菌样本中分离RNA。
- 配套的稳定裂解缓冲液适于处理松针或葡萄叶片等高难度样本。
- 可从大分子量mRNA、microRNA和病毒RNA之中分离出多种RNA。
- 可纯化出高得率、优质的RNA。
- 无需进行苯酚/氯仿萃取。
- 无需采用液态进行均质化
Other Notes
存储条件和产品稳定性
所有溶液应严格密封并在室温下储存。在未开封条件下，此类试剂至少可稳定保存1年。
所有溶液应严格密封并在室温下储存。在未开封条件下，此类试剂至少可稳定保存1年。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。
signalword
Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2
存储类别
10 - Combustible liquids
实验方案
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.
Yuan Song et al.
Biochemical and biophysical research communications, 396(2), 187-192 (2010-03-25)
DNA methyltransferase2 (DNMT2) is always deemed to be enigmatic, because it contains highly conserved DNA methyltransferase motifs but lacks the DNA methylation catalytic capability. Here we show that Arabidopsis DNA methyltransferase2 (AtDNMT2) is localized in nucleus and associates with histone
Michaela Novodvorska et al.
BMC genomics, 14, 246-246 (2013-04-13)
Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next generation RNA-sequencing and GeneChips. The metabolism of storage compounds during conidial germination was also examined and compared to the transcript levels from associated
Jun-Zhi Wen et al.
PloS one, 9(2), e88179-e88179 (2014-02-12)
MicroRNAs (miRNAs) are important regulators of many cellular processes and exist in a wide range of eukaryotes. High-throughput sequencing is a mainstream method of miRNA identification through which it is possible to obtain the complete small RNA profile of an
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E4913-1KT | 04061833604076 |