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Merck
CN

G7663

Gelatin blocking buffer

for Western blotting, powder blend

别名:

blocking buffer

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关于此项目

NACRES:
NA.25
UNSPSC Code:
41105300
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grade

Molecular Biology

Quality Level

form

powder blend

storage temp.

room temp

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Application

Suitable for use as a non-specific blocking agent for Western blots.

Preparation Note

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.


存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

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实验方案

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

为了特异性地检测固定在固体支持物上的抗原或靶分子,必须将支持物上未被占据的结合位点进行封闭以防止探针和检测分子结合。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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全球贸易项目编号

货号GTIN
G7663-1L04061833643068