ECL^™ Detection Reagents

The world’s first commercially available chemiluminescent detection reagent for Western blotting. Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

Based on the enhanced chemiluminescent reaction of luminol with horseradish peroxidase, ECL substrate can be used to detect probes which have been labelled either directly or indirectly with horseradish peroxidase. Rapid light output enables results to be achieved in 10 to 15 minutes. It is the substrate of choice for target amounts above 500 fg.

High sensitivity and fast results:

• Detects up to 10 to 12 ng of protein, which is ~10× more than colorimetric methods
• Optimized to use with Amersham Protran nitrocellulose (NC) membranes
• Delivers results in just a few minutes, then image using a CCD imager like the Amersham ImageQuant 800 or Amersham Hyperfilm ECL

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

ECL is a trademark of Cytiva