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Merck
CN

M8662

矿物油

PCR Reagent

别名:

Mineral oil for PCR

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关于此项目

化学文摘社编号:
UNSPSC Code:
12181504
NACRES:
NA.52
EC Number:
232-455-8
MDL number:
Technique(s):
PCR: suitable
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Quality Level

form

liquid

feature

PCR Additives

packaging

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

General description

矿物油可用作聚合酶链反应(PCR) 中的反应混合物覆盖层,以防止在没有加热盖的 PCR 仪器的热循环过程中反应溶液蒸发。

Application

矿物油已用于:
  • 常规PCR扩增
  • 逆转录聚合酶链反应 (RT–PCR) 扩增工艺,提高设备和循环仪之间的导热性
  • 防止在 Bravo 自动液体处理平台上进行的单细胞 MATQ 测序过程中蒸发

Features and Benefits

  • 以5 × 1.5 mL(1 瓶)包装规格提供,便于使用
  • 经测试不含 RNase、DNase和蛋白酶。


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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

ppe

Eyeshields, Gloves



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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