A1054
抗-地高辛-碱性磷酸酶抗体，小鼠单克隆
clone DI-22, purified from hybridoma cell culture
别名:
单克隆抗-地高辛, 单克隆抗 地高辛 – 碱性磷酸酶 小鼠抗
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关于此项目
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
DI-22, monoclonal
Application:
DB, ELISA (d)
Citations:
8
biological source
mouse
Quality Level
recombinant
expressed in mouse cell line
conjugate
alkaline phosphatase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
DI-22, monoclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate, direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate, dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
地高辛是一种强心苷，可用于治疗慢性心力衰竭。抗地高辛碱性磷酸酶单克隆抗体可用于检测地高辛标记的化合物，例如寡核苷酸、抗体和肽。该抗体对地高辛和地高辛标记的化合物具有特异性。该产品与地高辛具有很强的交叉反应性。
Immunogen
地高辛-KLH。
Application
巢式PCR产物可与5′端地高辛标记的寡核苷酸探针杂交。 向1 M磷酸盐缓冲液、1％牛血清白蛋白和0.1％吐温20（pH 7.4）中添加1:1000稀释的抗地高辛单克隆抗体碱性磷酸酶（DI-22）进行ELISA分析，以检测探针。使用Sigma Fass-Pnpp底物片剂进行检测。
抗地高辛碱性磷酸酶单克隆抗体适用于直接ELISA（1:10000-1:6000）和斑点杂交（以1:20,000稀释，使用地高辛标记的生物分子）。它还用于酶免疫分析（EIA）。
Biochem/physiol Actions
抗体对地高辛具有特异性，且对地高辛具有高亲和力。
Physical form
0.05 M Tris缓冲液，pH 8.0，含1% BSA、50%甘油、1 mM MgCl2和15 mM叠氮化钠
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
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J N Mbatha et al.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| A1054-.2ML | 04061833334737 |
| A1054-.5ML | 04061837854774 |