Merck
CN

B0519

Sigma-Aldrich

生物素−琼脂糖

PBS suspension

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别名:
维生素H琼脂糖
MDL编号:
NACRES:
NA.56

形式

PBS suspension

标记范围

≥30 mg avidin per mL packed resin
2-6 μmol per mL

基质

4% beaded agarose

基质活化

epoxy

基质附着

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

基质隔离区

13 atoms

储存温度

2-8°C

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应用

生物素含有与抗生物素蛋白或链霉亲和素结合的高亲和力。它在生物化学和分子生物学中已被广泛用于检测和标记蛋白质。

外形

混悬剂为含有0.02%叠氮化钠的磷酸盐缓冲盐水

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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K J Airenne et al.
Gene, 144(1), 75-80 (1994-06-24)
A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
A P Sibler et al.
Journal of immunological methods, 224(1-2), 129-140 (1999-06-05)
In vivo biotinylation of antibody fragments with a gene fusion approach is a realistic alternative to conventional in vitro chemical labelling. We have previously reported the construction of a vector system suitable for the bacterial expression of the binding fragment
Emrah Celik et al.
Journal of molecular recognition : JMR, 25(1), 53-56 (2012-01-04)
Sample-probe contact duration (dwell time) and loading force are two important parameters for the atomic force microscopy (AFM) force spectroscopy measurements of ligand-receptor interaction. A prolonged contact time may be required to initiate ligand-receptor binding as a result of slow
P A Smith et al.
Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet, N., et al.
Journal of Materials Chemistry, 14, 2638-2642 (2004)

相关内容

利用亲和力或GST沉降、串联亲和纯化(TAP)和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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