biological source
goat
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
technique(s)
immunoelectrophoresis: suitable
storage temp.
2-8°C
target post-translational modification
unmodified
General description
生物素被认为是一种必需的营养物质,尽管已知它是由一些肠道细菌分泌的。它微量存在于肝和脑组织中。
生物素:抗生物素蛋白第二抗体信号系统涉及生物素与第二抗体的偶联。一旦第二抗体在免疫化学测定中结合靶一抗,通过允许与酶或信号分子偶联的亲和素与抗体相关生物素分子结合来产生信号。
通过 Ouchterlony 双扩散和免疫电泳与生物素载体蛋白复合物测定特异性和反应性。未观察到与载体蛋白的反应性。
Immunogen
生物素-KLH 结合物
Application
可能取代亲和素以减少非特异性结合。
在一些应用中,生物素化探针与亲和素的定位会产生高背景水平。抗生物素试剂可替代亲和素,以减少非特异性结合。
山羊抗生物素抗体已用于多孔板试验,以鉴定脱硫生物素蛋白。此抗体还可用于色度酶免疫测定。
Biochem/physiol Actions
生物素可作为氧化代谢所需羧化酶的辅因子。摄入富含抗生物素蛋白的食物(如蛋白)会减少小肠中生物素的吸收。因此,显著降低其生物利用度,导致蛋白损伤(egg white injury)。妊娠期间通常可观察到生物素水平的下降。生物素缺乏症可能是先天的,缺陷蛋白涉及生物素稳态。
Physical form
从 0.01 M 磷酸钠、15 mM 氯化钠 (pH 7.2) 冻干
Preparation Note
用 0.135 M 氯化钠复溶。
Disclaimer
除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Marcus Braun et al.
Nature cell biology, 13(10), 1259-1264 (2011-09-06)
Short regions of overlap between ends of antiparallel microtubules are central elements within bipolar microtubule arrays. Although their formation requires motors, recent in vitro studies demonstrated that stable overlaps cannot be generated by molecular motors alone. Motors either slide microtubules
Zdenek Lansky et al.
Cell, 160(6), 1159-1168 (2015-03-10)
Cytoskeletal remodeling is essential to eukaryotic cell division and morphogenesis. The mechanical forces driving the restructuring are attributed to the action of molecular motors and the dynamics of cytoskeletal filaments, which both consume chemical energy. By contrast, non-enzymatic filament crosslinkers
Vito Cataldo et al.
Electroanalysis, 20(2), 115-122 (2008-10-11)
Amperometric immunosensor configurations featuring covalently bound anti-biotin antibodies (Ab) embedded into a polylysine (PLL)-single walled carbon nanotube (SWCNT) composite layer were evaluated. Assemblies were made by first oxidizing pyrolytic graphite (PG) electrodes to form surface carboxylic acid groups, to which
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| B3640-1MG | 04061833428290 |