Merck
CN

E3403

Millipore

EZview 红色蛋白 G 亲和凝胶

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MDL编号:
NACRES:
NA.56

形式

liquid

分析物化学类别

proteins (Immunoglobulins of various mammalian species)

技术

affinity chromatography: suitable
immunoprecipitation (IP): suitable

容量

≥8 mg/mL, gel binding capacity (rabbit IgG)

储存温度

−20°C

一般描述

EZ view Red蛋白G亲和凝胶通过共价键与4%琼脂糖珠结合。该亲和凝胶用于将抗体IgG的FC部分与样品结合,如细胞裂解物和组织,纯化标记蛋白以进行免疫沉淀分析。红色染料增强了可视性,以获得更有效的结果。

应用

在小规模亲和捕获中,如免疫沉淀,难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂,会导致结果产生数量差异。EZview 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同,但更易于区分沉淀和上清液。 这将获得更准确的数据,因为蛋白质损失减少。

特点和优势

  • 可见性增加-红色可降低意外吸入的风险
  • 通过减少意外损失提高目标蛋白的回收率
  • 重现性更高-收率更一致

外形

1:1(v/v)混悬液溶于含有50%甘油和15 ppm Kathon的PBS中。
PBS中50%悬浮液加入50%甘油及0.0015%

法律信息

EZview is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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