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安全信息

F4049

Sigma-Aldrich

FLAG® M2-FITC单克隆抗体 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous solution

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别名:
单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk
NACRES:
NA.32

生物来源

mouse

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution

种属反应性

all

浓度

~1 mg/mL

技术

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

FLAG M2-FITC被用于检测Flag融合蛋白。该单克隆抗体来自于小鼠，并与异硫氰酸荧光素（FITC）共价结合。该抗体可识别N末端、Met N末端和C末端的FLAG序列。结合不依赖于钙。F4049是一种亲和纯化的抗体，在大多数应用中可提高灵敏度。

应用

抗体适用于免疫荧光、免疫细胞化学、免疫组织化学、免疫印迹、流式细胞术和电子显微镜分析。该抗体特别适用于检测在小鼠宿主中表达的Flag融合蛋白，其中二抗可能引起交叉反应。

请访问我们的 FLAG® 文献 门户网站，浏览其他应用参考文献。

外形

溶于10 mM磷酸盐缓冲盐水，含有1% BSA和0.1%叠氮化钠，pH 7.4

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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