Merck
CN

H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

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NACRES:
NA.75

无菌性

sterile-filtered

产品线

BioReagent

形式

liquid

技术

cell culture | hybridoma: suitable
single cell analysis: suitable

杂质

endotoxin, tested

密度

1.077 g/cm3 at 25 °C (lit.)

储存温度

2-8°C

一般描述

Histopaque®-1077是包含多蔗糖和泛影酸钠的溶液,密度调整到1.077 +/- 0.001 g/cm3这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原(HLA)2分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基,用于淋巴细胞和其他单核细胞的纯化。

应用

Histopaque®-1077 Hybri-Max已用于分离:
  • 外周血单个核细胞(PBMC)与肝素化血,梯度离心法
  • 来自外周血的免疫细胞(淋巴细胞)
  • PBMC与血液

法律信息

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

动植物来源培养基

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Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(2), 160-167 (2008-01-15)
Coulter volume is far more accurate measure of cell volume than forward angle light scatter. In this report, we have used Coulter volume to determine the mean cell volume and diameter of normal human peripheral blood cells and hematopoietic progenitor
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As in human populations, advances in nutrition and veterinary care have led to an increase in the lifespan of companion animals. Detrimental physiological changes occurring later in life must be understood before interventions can be made to slow or reduce
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