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文件

安全信息

I1146

Sigma-Aldrich

R3 IGF-1 人

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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NACRES:
NA.77

生物来源

human

质量水平

200

重组

expressed in E. coli

检测方案

≥85% (HPLC)

形式

lyophilized powder

效能

≤10 ng/mL ED50

质量

endotoxin tested

分子量

7,676 Da

包装

pkg of 1 mg

技术

cell culture | mammalian: suitable

UniProt登记号

P05019

储存温度

2-8°C

基因信息

human ... IGF1(3479)

应用

R3 IGF-I 是胰岛素样生长因子的重组类似物，包含完整的人 IGF-I 氨基酸序列，其中 Arg 取代了 Glu3。它被开发为一种廉价、高质量、高效的 IGF 类似物，可用作无血清或低血清细胞培养的生长因子补充剂。

使用专利表达系统在大肠杆菌中重组生产，该肽是从溶解并脱盐的包含体的细胞中收获。活性分子在氧化条件下适当折叠，然后通过多个液相色谱步骤纯化。

生化/生理作用

产品刺激 L6 成肌细胞中的蛋白质合成。

其他说明

胰岛素样生长因子-I 的重组类似物，在第 3 位用 Arg 取代 Glu。

制备说明

由0.1 M醋酸制备

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
胰岛素激素类产品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?

    The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Peter Tontonoz et al.
Molecular and cellular biology, 35(7), 1125-1138 (2015-01-22)
We previously showed that the orphan nuclear receptor Nur77 (Nr4a1) plays an important role in the regulation of glucose homeostasis and oxidative metabolism in skeletal muscle. Here, we show using both gain- and loss-of-function models that Nur77 is also a

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