Merck
CN

I2644

Sigma-Aldrich

白细胞介素-2 人

IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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别名:
IL-2, hIL-2, T细胞生长因子, 白细胞介素-2
MDL编号:
NACRES:
NA.77

生物来源

human

质量水平

重组

expressed in E. coli

形式

lyophilized powder

效能

≤700 EC50

质量

endotoxin tested

分子量

15.5 kDa

包装

pkg of 5X10 μg
pkg of 10 μg

技术

cell culture | mammalian: suitable

杂质

≤1 EU/μg

UniProt登记号

储存温度

−20°C

基因信息

human ... IL2(3558)

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一般描述

白细胞介素-2 (IL-2)是一种α螺旋状细胞因子,属于一型T辅助细胞。它的分子量为15kDa。IL-2 基因位于人染色体 4q27。IL-2 最初被称为T细胞生长因子。

应用

人白细胞介素-2 已被用于:
  • 模拟自然杀伤细胞
  • 研究其刺激淋巴细胞生长和防止细胞凋亡的能力
  • 作为酶联免疫吸附测定 (ELISA) 中的抗原,用于分析一型糖尿病中人体自身抗体反应活性。

生化/生理作用

白细胞介素-2 (IL-2)对于自然杀伤(NK)细胞淋巴细胞和巨噬细胞的增生至关重要。它在免疫系统功能(如NK细胞刺激的免疫反应)中发挥关键作用。IL-2也介导调节性T细胞的基本功能。它与自体免疫疾病有关。IL-2与细胞凋亡有关,因此具有抗肿瘤反应。它介导CD41+ T-细胞分化成辅助细胞1 和 2 效应细胞亚类,从而防止T辅助细胞17 分化。
白细胞介素-2(IL-2)是由某些T淋巴细胞亚群产生的免疫调节因子。该淋巴因子能够促进活化T细胞和相关细胞类型的长期生长。IL-2在NK细胞的激活和增殖、诱导γ-干扰素和B细胞生长因子分泌中发挥作用,并且能够调节IL-2受体的表达。

外形

冻干品,经0.2 μm滤膜过滤,无添加剂。

分析说明

通过剂量依赖性刺激鼠CTLL-2细胞的增殖来测量生物活性。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Where can I find information on the specific activity of Product I2644, Interleukin-2 human - recombinant?

    The EC50 has been determined by dose-dependent stimulation of the proliferation of murine CTLL-2 cells.  You can find the lot specific value for the EC50 of this product on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on the product detail page (for I2644).

  4. Is Product I2644, Interleukin-2 human - recombinant, endotoxin tested?

    Yes, the endotoxin levels are reported on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on the product page (for I2644).

  5. How can I convert the EC50 of interleukin from ng/mL to units/mL when using Product I2644, Interleukin-2 human - recombinant?

    The specific activity in Units/mg = 1 × 106 / ED50 (in ng/ml).

  6. How should I dissolve Product I2644, Interleukin-2 human - recombinant?

    Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers.

  7. How should solutions of Product I2644, Interleukin-2 human - recombinant, be stored?

    Interleukin solutions can be stored at 2-8°C for up to 1 week. For extended storage, freeze at -20°C in working aliquots to avoid freezing and thawing.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Loss of immune tolerance to IL-2 in type 1 diabetes
Perol L, et al.
Nature Communications, 7(2), 13027-13027 (2016)
Culture methods for turtle lymphocytes
Ulsh BA, et al.
Methods in Cell Science : An Official Journal of the Society for In Vitro Biology, 22(4), 285-297 (2000)
Natural killer cells in the synovial fluid of rheumatoid arthritis patients exhibit a CD56bright, CD94bright, CD158negative phenotype
Pridgeon C, et al.
Rheumatology (Basel), 42(7), 870-878 (2003)
Francisco J Quintana et al.
Journal of autoimmunity, 21(1), 65-75 (2003-08-02)
Informatic methodologies are being applied successfully to analyze the complexity of the genome. But beyond the genome, the immune system reflects the state of the body in health and disease. Traditionally, immunologists have reduced the immune system, where possible, to
Interleukin 2, 37-37 (2012)

商品

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The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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