L6632
脂肪氧化酶 来源于大豆
Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein
别名:
亚油酸：氧氧化还原酶, 脂氧合酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-853-1
MDL number:
EC 号:
Specific activity:
500,000-1,000,000 units/mg protein
type
Type V
Quality Level
form
ammonium sulfate suspension
specific activity
500,000-1,000,000 units/mg protein
mol wt
94 kDa
storage temp.
2-8°C
Application
大豆酶将使用花生四烯酸作为底物，以亚油酸作为底物的活性约为 15%；花生四烯酸氧化的产物是 12-或 15-过氧化氢花生四烯酸（12-HPETE 或 15-HPETE）。
来自Glycine max （大豆）的脂氧酶（也称脂肪氧合酶）已被用于修饰从人血浆中分离的低密度脂蛋白。
Biochem/physiol Actions
催化含有顺式，顺式-1,4-戊二烯结构的脂质的氢过氧化。
Physical form
悬浮于2.3 M（NH4）2SO4溶液中，pH约为6.0
Preparation Note
通过离子交换色谱和疏水相互作用色谱法制备。
Analysis Note
通过缩二脲法测定的蛋白质。
Other Notes
一个酶活性单位是指在pH 9.0、25°C 下，在3.0 ml体积（1 cm光程）中使用亚油酸作为底物时，每分钟使A234 增加0.001所需的酶量。一个A234 单位相当于μmole亚油酸的氧化。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
P Harduin et al.
Journal of lipid research, 36(5), 919-930 (1995-05-01)
We studied the effect of in vitro moderate oxidation on low density lipoprotein (LDL) conformation and metabolism. LDL was modified with either copper ions or phospholipase A2 plus lipoxygenase and, in both cases, mild oxidative conditions were used. The resulting
Marc P Baggelaar et al.
Bioorganic & medicinal chemistry, 21(17), 5271-5274 (2013-07-23)
A catalytic asymmetric synthesis of (S)-(-)-zearalenone is reported using asymmetric allylic alkylation for the introduction of the stereocenter. (S)-(-)-Zearalenone turned out to be a novel lipoxygenase inhibitor.
Xinyao Lu et al.
Applied microbiology and biotechnology, 97(21), 9419-9427 (2013-03-16)
Self-assembling amphipathic peptides (SAPs) are a category of peptides that have unique sequences with alternating hydrophobic and hydrophilic residues that can spontaneously assemble into ordered nanostructures. In this study, we investigated the potential of fusion technique with SAPs to improve
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L6632-1MU | 04061833958933 |
| L6632-5MU | 04061833621318 |