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M0275

Eagle最低必需培养基

Name: Eagle最低必需培养基
Brand: SIGMA

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

别名:

MEM

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关于此项目

UNSPSC Code:

12352207

NACRES:

NA.71

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属性

产品名称

Eagle最低必需培养基, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO₂): yes
L-glutamine: no
sodium pyruvate: no
NaHCO₃: no

shipped in

ambient

storage temp.

2-8°C

说明

Application

最低必需培养基 Eagle 已用于配制甲基丙烯酸丙烯酰胺（水凝胶）。它也已用于培养牛膝关节的软骨，随后该牛膝关节软骨可用于 MRI（磁共振成像）研究和组织学分析。

通常用于在FBS、小牛或马血清存在下培养贴壁细胞，如成纤维细胞。待培养基稀释到1x和调节pH后，再添加L-谷氨酰胺和碳酸氢钠。

Preparation Note

向 1× 培养基中添加 0.292g/L L-谷氨酰胺、2.2g/L 碳酸氢钠。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.