M8069
Monoclonal Anti-Mad1 antibody produced in mouse
~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Mitotic Arrest Deficient
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关于此项目
Conjugate:
unconjugated
Clone:
9B10, monoclonal
Application:
ARR, ICC, IP
Citations:
6
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9B10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~85 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 10-20 μg/mL using HeLa cells, immunoprecipitation (IP): suitable, microarray: suitable
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAD1L1(8379)
General description
Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.
Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.
Immunogen
recombinant human Mad1 protein.
Application
Monoclonal Anti-Mad1 antibody produced in mouse has been used in
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).
Physical form
Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Jun Wan et al.
Current biology : CB, 24(22), 2687-2692 (2014-12-03)
Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the major cell-cycle checkpoint that regulates chromosome segregation during mitosis, the mitotic checkpoint (also known as the spindle assembly checkpoint). During mitosis, Mad1 recruits Mad2 to unattached kinetochores, where Mad2
Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex.
Gang Zhang et al.
The EMBO journal, 38(7) (2019-02-21)
Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist.
Garry G Sedgwick et al.
mAbs, 8(4), 689-697 (2016-03-18)
The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly to Cdc20
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M8069-200UL | 04061837868146 |