Monoclonal Anti-phospho-MDMX (pTyr⁹⁹) 小鼠抗

MDMX is a nuclear protein and the gene encoding it is localized on human chromosome 1q32.1.

Monoclonal Anti-phospho-MDMX (pTyr99) (mouse IgG2b isotype) is derived from the hybridoma PH-MDMX-169 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse double minute 4 (MDM4) or MDMX comprises p53 binding domain, central acidic region, zinc and interesting new gene (RING) finger domain and is a phosphorylated protein. MDM4 gene is mapped to human chromosome 1q32.1.

synthetic phosphopeptide corresponding to amino acids 93-106 (pTyr⁹⁹) of human MDMX.

Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunofluorescence
• immunoblotting

Studies began to unravel the regulation of murine double minute X (MDMX) in response to stress conditions, involving post-translational modifications and protein-protein interactions.

MDMX associates with tumor suppressor p53 and inhibits its activity. It is upregulated in many tumors.

Monoclonal Anti-phospho-MDMX (pTyr99) recognizes human phosphorylated MDMX (pTyr99) (~ 80 kDa). The antibody does not recognize the non-phosphorylated MDMX, which is produced when MDMX is expressed with a kinase mutant.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

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