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M9004

苹果酸脱氢酶 来源于牛心脏

ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

别名:

L -苹果酸盐：NAD + 氧化还原酶, MDH

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关于此项目

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
MFCD00131567
EC 号:
1.1.1.37 (BRENDA, IUBMB)
Specific activity:
2000-4000 units/mg protein (modified Warburg-Christian)
Biological source:
bovine heart
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biological source

bovine heart

Quality Level

200

form

ammonium sulfate suspension

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

mol wt

35 kDa

impurities

≤0.01% Glutamic-Oxalacetic Transaminase, ≤0.01% Glutamic-Pyruvic Transaminase

storage temp.

2-8°C

General description

牛心脏的苹果酸脱氢酶在 NAD 结合活性位点包含一个组氨酸残基，该残基对活性至关重要。当该组氨酸突变时，观察到活性丧失。
苹果酸脱氢酶是一种细胞质同工酶，是三羧酸循环中的重要催化剂。

Application

苹果酸脱氢酶已用于评估流动注射系统的研究，用于在线监测生物过程中的富马酸。 它也曾被用于一项研究，调查一种寄生在德州花生上的根结线虫。

Physical form

3 M (NH4)2SO4 - 0.01 M KH2PO4 溶液 (pH 7.3) 中的混悬液

Other Notes

在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmol的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCH6610
SLCB1651
098K7680V
098K7680
058H78041

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实验方案

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

商品

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以硫酸铵悬浮液形式提供的酶的使用指南

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Enika Nagababu et al.
Methods in molecular biology (Clifton, N.J.), 610, 165-180 (2009-12-17)
Reactive oxygen species are implicated in many human diseases and aging process. Much of the evidence is based on experimental data indicating increasing rates of lipid peroxidation in disease states and the ameliorating effects of antioxidants. It is becoming increasingly
E M Gregory
The Journal of biological chemistry, 250(14), 5470-5474 (1975-07-25)
Bovine mitochondrial malate dehydrogenase (EC 1.1.1.37) was inactivated by the specific modifications of a single histidine residue upon reaction with iodoacetamide. NADH protected against this loss of activity and reaction with the histidine residue, suggesting that the histidine is at
Flow injection system for on-line monitoring of fumaric acid in biological processes
Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)
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全球贸易项目编号

货号GTIN
M9004-5KU04061834063797
M9004-10KU04061834063780
M9004-25KU04061832731001