Merck
CN

N6658

Sigma-Aldrich

营养混合物 F-12 Ham

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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别名:
Ham's F-12
MDL编号:
NACRES:
NA.75

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

运输

ambient

储存温度

2-8°C

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一般描述

Ham′s营养混合物主要是为支持中国仓鼠卵细胞、HeLa细胞和小鼠L细胞的克隆生长而研制。也可用于原代大鼠肝细胞和大鼠前列腺上皮细胞的生长。Ham′s F-12是基于CHO细胞的克隆毒性分析的常用培养基。

应用

Ham F-12营养混合物已用于:
  • 培养过表达胰岛素受体的中国仓鼠卵巢细胞(CHO-IR)
  • 培养LAN-1细胞
  • 维持培养SUM149、SUM159、SUM190、FC-IBC02、BCX010和IBC3细胞

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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