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P3391

蛋白 A-琼脂糖™ 来源于金黄色葡萄球菌

lyophilized powder

别名:

蛋白 A-琼脂糖

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关于此项目

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
MFCD00165563
Form:
lyophilized powder
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form

lyophilized powder

Quality Level

200

packaging

glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g

storage condition

do not freeze

extent of labeling

3 mg per mL

technique(s)

affinity chromatography: suitable

color

white to gray

matrix

Sepharose CL-4B

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥16 mg/mL binding capacity (human IgG)

solubility

water: 1 g/L at 20 °C

suitability

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

storage temp.

2-8°C

General description

当与抗体连接时，蛋白A-琼脂糖凝胶有助于提高交联到柱基质上的IgG的抗原结合能力。在这种情况下，结合发生在蛋白A与IgG分子的Fc部分之间，使抗原特异性位点游离。

Application

蛋白A-琼脂糖凝胶™用于亲和层析、抗体纯化和表征、免疫亲和基质、蛋白层析、蛋白A、G和L树脂以及重组蛋白表达和分析。蛋白A-琼脂糖凝胶™已用于制定研究蛋白相互作用的策略，改善乳糜泻检测，以及研究儿童糖尿病。
蛋白A-琼脂糖凝胶具有以下用途：
  • 用于免疫沉淀胎盘，使用蛋白印迹技术进行蛋白质和RNA分析。
  • 用于染色质免疫沉淀，以了解介体和特定启动子染色质结构重构之间的联系。
  • 用作裂解缓冲液，以去除雌性大鼠脑样本蛋白质提取中的内源性G型Ig。
  • 用于兔抗血清的[32P]磷肉豆蔻素C的免疫沉淀反应，以研究蛋白激酶C （PKC）和磷肉豆蔻素C （PMC）在T细胞发育过程中的下调。

Physical form

以用乳糖和葡聚糖稳定的冻干粉形式供应。

Preparation Note

膨胀：1 g膨胀至约4 ml。

Legal Information

Sepharose is a trademark of Cytiva

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBX9421
SLCF8184
SLBR6283V
SLBJ4075V
SLBL6140V

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Product Information Sheet - P3391
Plant Reagents
Lionel Gissot et al.
Plant physiology, 142(3), 931-944 (2006-10-10)
The sucrose nonfermenting-1 protein kinase (SNF1)/AMP-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, the beta- and gamma-noncatalytic subunits are implicated in substrate specificity and subcellular localization
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
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全球贸易项目编号

货号GTIN
P3391-5G04061834367819
P3391-1.5G04061834367802
P3391-1G04061835573851
P3391-250MG04061835512874