P3752
酸性磷酸酶 来源于马铃薯
lyophilized powder, ≥0.5 unit/mg solid
别名:
(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶 来源于马铃薯
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关于此项目
化学文摘社编号:
eCl@ss:
42010102
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-630-9
MDL number:
Specific activity:
≥0.5 unit/mg solid
Biological source:
potato
biological source
potato
Quality Level
form
lyophilized powder
specific activity
≥0.5 unit/mg solid
mol wt
69 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
SMILES string
O(CC(O)C)CC#C
InChI
1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
InChI key
GZCWLCBFPRFLKL-UHFFFAOYSA-N
Application
酸性磷酸酶通常用于蛋白质和核酸的去磷酸化。 酸性磷酸酶是一种酸性水解酶，通常存在于溶酶体中。它可作为鉴定亚细胞组分中溶酶体的标记物。 酸性磷酸酶（产品P3752）已被作为对照，用于研究 牛分枝杆菌 BCG如何影响肺泡巨噬细胞中酸性磷酸酶的活性水平。
Biochem/physiol Actions
酸性磷酸酶是一种细胞质溶酶体水解酶，可催化正磷酸单酯和水转化为醇和正磷酸盐。
Other Notes
在pH 4.8，37 °C条件下，一个单位可在每分钟水解1.0 μmole 对硝基苯磷酸盐。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
在测定酸性磷酸酶活性时，本操作流程使用对硝基苯基磷酸盐进行比色法测定。酶活性是使用分光光度计在410 nm处测定的。
Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)
K Zyła et al.
The British journal of nutrition, 74(1), 3-17 (1995-07-01)
An in vitro method was developed to predict inorganic P release from maize-soyabean poultry feeds containing supplemental phytase (EC 3.1.3.8), and to quantify the effect of acid phosphatase (EC 3.1.3.2), fungal protease (EC 3.4.23.6) and Aspergillus niger cellulase (EC 3.2.1.4)
I N Mbawuike et al.
Infection and immunity, 51(2), 483-489 (1986-02-01)
The kinetics of induction of the bronchoalveolar cell population (i.e., alveolar macrophages [AM], lymphocytes, and polymorphonuclear leukocytes) was studied in mice inoculated intravenously with heat-killed Mycobacterium bovis BCG. Injection of BCG at 100 and 500 micrograms but not at 10
A Waheed et al.
The EMBO journal, 7(8), 2351-2358 (1988-08-01)
BHK cells transfected with human lysosomal acid phosphatase (LAP) cDNA (CT29) expressed 70-fold higher enzyme activities of acid phosphatase than non-transfected BHK cells. The CT29-LAP was synthesized in BHK cells as a heterogeneously glycosylated precursor that was tightly membrane associated.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P3752-100MG | 04061834372837 |
| P3752-1G | 04061835506552 |