P8038
细菌蛋白酶
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
别名:
蛋白酶 来源于地衣芽孢杆菌, 枯草肽酶A, 碱性蛋白酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.54
MDL number:
Specific activity:
≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid
type
Type XXIV
Quality Level
form
lyophilized powder
specific activity
≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid
mol wt
27 kDa
purified by
crystallization
shipped in
wet ice
storage temp.
−20°C
General description
枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。
Application
来自sigma的该酶已用于优化大鼠心脏均质化心室组织中所有线粒体群的释放。它还用于福尔马林固定石蜡包埋肝脏样本的预杂交处理，用于人和病毒DNA的检测。
这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶，并在碱性条件下具有活性。 产品P8038，也被称为枯草杆菌蛋白酶Carlsberg，已用于水解心肌细胞以研究心脏线粒体NHE1的沉默。
Biochem/physiol Actions
枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白，并在碱性条件下具有活性。
蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活，如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。
Analysis Note
氨基酸分析和等电聚焦电泳与枯草杆菌蛋白酶Carlsberg一致。
Other Notes
在pH 7.5，37 °C 条件下，一单位的酶每分钟将会水解酪蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
10 - Combustible liquids
wgk
WGK 2
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
常规特殊物品
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8038-1G | 04061834400875 |
| P8038-50MG | 04061834400899 |
| P8038-100MG | 04061834400868 |
| P8038-250MG | 04061834400882 |