Merck
CN

P9424

Millipore

ProteinA-Sepharose 4, Fast Flow 来源于金黄色葡萄球菌

aqueous ethanol suspension

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别名:
蛋白 A-琼脂糖
MDL编号:
NACRES:
NA.56

生物来源

Staphylococcus aureus

形式

aqueous ethanol suspension

分析物化学类别

proteins (Immunoglobulins of various mammalian species)

标记范围

~6 mg per mL

技术

affinity chromatography: suitable

基质

Sepharose 4B Fast Flow

基质活化

cyanogen bromide

基质附着

amino

基质隔离区

1 atom

容量

≥30 mg/mL binding capacity (human IgG)

储存温度

2-8°C

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一般描述

Protein A-Sepharose已用于制定蛋白质相互作用研究策略、改进乳糜泻的检测,以及研究儿童糖尿病。

应用

来自金黄色葡萄球菌的Protein A-Sepharose Fast Flow已用于
  • i免疫共沉淀检测
  • 免疫耗竭
  • 从人血清和血浆中纯化IgG

蛋白A-琼脂糖凝胶用于亲和层析、抗体纯化和表征、免疫亲和基质、蛋白层析、蛋白A、G和L树脂以及重组蛋白表达和分析。蛋白A-琼脂糖凝胶已用于制定研究蛋白相互作用的策略,改善乳糜泻检测,以及研究儿童糖尿病。

外形

20% 乙醇混悬液

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°C)

40 °C

法规信息

常规特殊物品

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J Ziebuhr et al.
The Journal of biological chemistry, 276(35), 33220-33232 (2001-06-30)
The largest replicative protein of coronaviruses is known as p195 in the avian infectious bronchitis virus (IBV) and p210 (p240) in the mouse hepatitis virus. It is autocatalytically released from the precursors pp1a and pp1ab by one zinc finger-containing papain-like
C Macaulay et al.
The Journal of biological chemistry, 270(1), 254-262 (1995-01-06)
During each cell cycle, the nucleus of higher eukaryotes undergoes a dramatic assembly and disassembly. These events can be faithfully reproduced in vitro using cell-free extracts derived from Xenopus eggs. Such extracts contain three major N-acetylglucosaminylated proteins, p200, p97, and
Mazhar Adli et al.
Nature protocols, 6(10), 1656-1668 (2011-10-01)
Chromatin immunoprecipitation (ChIP) combined with high-throughput sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions. However, conventional ChIP protocols necessitate the use of large numbers of cells, and library preparation steps associated with current high-throughput sequencing
H Sternlicht et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(20), 9422-9426 (1993-10-15)
A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled
Lydia Alvarez-Erviti et al.
Neurobiology of disease, 42(3), 360-367 (2011-02-10)
Alpha-synuclein aggregation plays a central role in Parkinson's disease pathology. Direct transmission of alpha-synuclein from pathologically affected to healthy unaffected neurons may be important in the anatomical spread of the disease through the nervous system. We have demonstrated that exosomes

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