P9614
λ 蛋白磷酸酶
liquid, Bacteriophage Lambda, recombinant, expressed in E. coli
别名:
λ 酶
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关于此项目
Recombinant:
expressed in E. coli
Concentration:
≥400000 units/mL
recombinant
expressed in E. coli
Quality Level
form
liquid
mol wt
25 kDa
concentration
≥400000 units/mL
shipped in
dry ice
storage temp.
−70°C
General description
λ 蛋白磷酸酶是一种在 大肠杆菌中表达的重组蛋白 。它需要 Mn 2 + 或 Ni 2 + 作为活化剂。
Biochem/physiol Actions
λ 蛋白磷酸酶已被用于对海拉细胞提取物进行去磷酸化。
可用于从蛋白质中的丝氨酸、苏氨酸或酪氨酸残基释放磷酸基团。对磷酸化组氨酸残基也有活性。
Other Notes
在 30℃，pH 7.5的条件下，一单位每分钟水解 1 nmol 对硝基苯磷酸盐。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
Zhuo S
The Journal of Biological Chemistry, 268(24), 17754-17761 (1993)
P T Cohen et al.
The Biochemical journal, 260(3), 931-934 (1989-06-15)
Infection of Escherichia coli with phage lambda gt10 resulted in the appearance of a protein phosphatase with activity towards 32P-labelled casein. Activity reached a maximum near the point of cell lysis and declined thereafter. The phosphatase was stimulated 30-fold by
Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.
Ishii T
The Journal of Biological Chemistry, 285(53), 41993-42000 (2010)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P9614-20KU | 04061834404170 |