R1003
核糖核酸酶T1 来源于米曲霉
ammonium sulfate suspension, 300,000-600,000 units/mg protein
别名:
核糖核苷-3′-脒基-寡核苷酸水解酶, 鸟粪核糖核酸酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-809-1
MDL number:
Specific activity:
300,000-600,000 units/mg protein
Biological source:
Aspergillus sp. (Aspergillus oryzae)
biological source
Aspergillus sp. (Aspergillus oryzae)
Quality Level
form
ammonium sulfate suspension
specific activity
300,000-600,000 units/mg protein
mol wt
11068 by amino acid sequence
technique(s)
cell based assay: suitable
suitability
suitable for separating native or denatured proteins, or nucleic acids
application(s)
cell analysis
storage temp.
2-8°C
Application
来自 米曲霉的核糖核酸酶T1（RNase T1）可用于在测序之前对变性的RNA进行消化并用于蛋白折叠的研究 。
Biochem/physiol Actions
来自米曲霉的核糖核酸酶T1（RNase T1）是一种内切核糖核酸酶，其可在G残基后进行水解。切割发生在胍核糖核苷酸的3'-磷酸基团和相邻核苷酸的5'-羟基之间。初始产物为2'：3'环磷酸核苷，其可水解成相应的3'-核苷磷酸。它与胰腺RNase的不同之处在于它可特异性攻击鸟嘌呤位点以产生具有3′-GMP末端基团的3′-GMP和寡核苷酸。
Physical form
2.8 M (NH4)2SO4 溶液混悬液
Analysis Note
E1%/280法测定蛋白
Other Notes
在pH7，5,37°C条件下，一个单位可在15分钟内产生相当于 ΔA260 为 1.0 的酸可溶性寡核苷酸，反应体积为1.0 mL。底物：酵母RNA。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Caroline Haupt et al.
Journal of the American Chemical Society, 133(29), 11154-11162 (2011-06-15)
Slow protein folding processes during which kinetic folding intermediates occur for an extended time can lead to aggregation and dysfunction in living cells. Therefore, protein folding helpers have evolved, which prevent proteins from aggregation and/or speed up folding processes. In
Elisa Bombarda et al.
The journal of physical chemistry. B, 114(5), 1994-2003 (2010-01-22)
Because of their central importance for understanding enzymatic mechanisms, pK(a) values are of great interest in biochemical research. It is common practice to determine pK(a) values of amino acid residues in proteins from NMR or FTIR titration curves by determining
Ribonuclease T1: Structure, Function, and Stability
Pace, CN; Heinemann U, et al.
Angewandte Chemie (International Edition in English), 4, 343-360 (1991)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R1003-500KU | 04061832759432 |
| R1003-100KU | 04061832759425 |