SRE0019
三磷酸腺苷双磷酸酶 来源于马铃薯
Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein
别名:
腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-569-8
MDL number:
Quality Level
type
Grade VII
form
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition
Protein, 25-60%
solubility
H2O: soluble 1.0 mg/mL
application(s)
diagnostic assay manufacturing
foreign activity
Acid Phosphatase ≤2% of base activity
storage temp.
−20°C
Application
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+Pi → AMP+2Pi。
反应：ATP → ADP+Pi → AMP+2Pi。
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Physical form
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Preparation Note
按 ATP 酶单位销售。
Other Notes
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
该等级具有低 ATP 酶/ADP 酶比率。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Kettlun, A., et al.
Phytochemistry, 21, 551-551 (1982)
Differentiation of platelet-aggregating effects of human tumor cell lines based on inhibition studies with apyrase, hirudin, and phospholipase.
E Bastida et al.
Cancer research, 42(11), 4348-4352 (1982-11-01)
David Riewe et al.
Plant physiology, 147(3), 1092-1109 (2008-05-16)
Apyrases hydrolyze nucleoside triphosphates and diphosphates and are found in all eukaryotes and a few prokaryotes. Although their enzymatic properties have been well characterized, relatively little is known regarding their subcellular localization and physiological function in plants. In this study
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SRE0019-100UN | 04061833226650 |
| SRE0019-1KU | 04061826704035 |
| SRE0019-200UN | 04061833226667 |
| SRE0019-2KU | 04061833226674 |
| SRE0019-500UN | 04061833226681 |