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Enzyme activity assays

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facet applications:Enzyme activity assays
facet content type:Technical Article
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Enzymatic Assay of Cathepsin B
This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.
Enzymatic Assay of Proteinase K with Hemoglobin Substrate
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
PKC
Sigma-Aldrich offers many products related to PKC for your research needs.
酶法测定淀粉酶活性(淀粉酶活性测定)
使用淀粉酶活性测定试剂盒比色检测生物样品中淀粉酶活性的测定实验方案。
如何使用溶于硫酸铵悬液的酶
以硫酸铵悬浮液形式提供的酶的使用指南
Nuclear Receptors (PPARs)
Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors.
Drug Conjugate Analysis using β-Glucuronidases
Drug conjugate analysis and the enzymatic hydrolysis of glucuronides
InsR
We offers many products related to InsR for your research needs.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Phospholipase D
Phosphatidylcholine (PC)-specific phospholipase D (PLD) enzymes catalyze the hydrolysis of PC to generate choline and phosphatidic acid. These enzymes belong to a superfamily of phosphatidyltransferases with a characteristic unique duplicated catalytic domain motif, the PLD or " HKD"
Excitatory Amino Acid Transporters
Sigma-Aldrich offers many products related to excitatory amino acid transporters for your research needs.
胆固醇酯化TEST
胆固醇酯化可改善运输。胆固醇酯可更轻松地封装在脂蛋白内部-增加了在血液中可轻松运输的胆固醇数量。
Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I.
Neurotensin Receptors
Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.
Phospholipase C
The hydrolysis of a minor membrane phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2) by a specific phospholipase C (PLC) is one of the earliest key events in the regulation of various cell functions by more than 100 extracellular signaling molecules.
Neuropeptidases
We offers many products related to neuropeptidases for your research needs.
β Secretase (BACE1) Activity Assay for BACE1 Inhibitor Screening
In recent years, the biological processes involved in the pathogenesis of Alzheimer’s disease (AD) have become increasingly better understood. It is now recognized that a key role is played by BACE1 proteases that cleave amyloid precursor protein (APP) to produce
Enzymatic Assay of Chitosanase from Streptomyces Species
To standardize a procedure for determining the enzymatic activity of Chitosanase.
HMG-CoA Reductase (HMGR) Assay Kit
3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.
Enzymatic Assay of Cathepsin B
Enzymatic Assay of Cathepsin B
Enzymatic Assay of Proteinase K with Hemoglobin Substrate
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
Enzymatic Assay of Deoxyribonuclease I (EC 3.1.21.1)
To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.
PKA & PKG
Our offers many products related to PKA & PKG for your research needs.
Excitatory Amino Acid Transporters
Sigma-Aldrich offers many products related to excitatory amino acid transporters for your research needs.
Bioluminescent Firefly Luciferase Assays
Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.
Phospholipase C
We offers many products related to phospholipase C for your research needs.
Enzymatic Assay of Proteinase K with Hemoglobin Substrate
Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol
G Proteins (Heterotrimeric)
We offers many products related to heterotrimeric G-proteins for your research needs.
PKC
Sigma-Aldrich offers many products related to PKC for your research needs.
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