Protocol

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Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

The Bio-Rad digital droplet system is based on oil emulsification technology and uses a standard 96-well plate format. Each sample is partitioned into 20,000 reactions, yielding a total of 1.9 million reactions per plate.

Viral RNA Purification Protocol Using GenElute™ Mammalian Total RNA Purification Kits

Instructions for purifying viral RNA using GenElute™ Mammalian Total RNA Purification Kits.

Buchwald-Hartwig Amination Reaction in Water at Room Temperature using TPGS-750-M

TPGS-750-M, a second generation surfactant, may be used in the Buchwald-Hartwig Amination Reaction in Water at Room Temperature.

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

Titan™ One Tube RT-PCR System Protocol & Troubleshooting

Titan One Tube RT-PCR System Protocol & Troubleshooting

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution puriﬁcation of GST-tagged proteins, providing sharp peaks and concentrated eluent.

脂多糖

脂多糖（LPS）是革兰氏阴性细菌细胞壁的特征成分；在革兰氏阳性细菌中不存在。它位于膜外层，在无包膜菌株中暴露于细胞表面。它们有助于维持外膜完整性，保护细胞免受胆汁盐和亲脂性抗生素的作用。

过氧化氢酶的酶学测定（EC 1.11.1.6）

本程序适用于所有过氧化氢酶产品。

Plasmid DNA Purification using illustra™ plasmidPrep Mini Spin Kit

This page shows Plasmid DNA purification using illustra™ plasmidPrep Mini Spin Kit from GE Healthcare.

质粒DNA共转染

质粒的瞬时共转染是一种细胞蛋白互作研究、转录因子研究和shRNA编码质粒基因敲减研究的常用方法。

Analytical Method: Lead in waste water with high concentrations of calcium

Photometric determination with PAR subsequent to separation of lead with Sodium diethyldithiocarbaminate.

醇脱氢酶（EC 1.1.1.1）的酶学测定

该程序可用于醇脱氢酶产物。

Preactivated Magnetic Beads for Affinity Chromatography

Covalent coupling of antibodies, aptamers, and proteins with a NHS Mag Sepharose, Sera-Mag Carboxylate-Modiﬁed, Sera-Mag SpeedBeads Carboxylate-Modiﬁed from GE Healthcare.

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

Step-by-Step Guide for KitAlysis™ High-Throughput Photocatalysis Reaction Screening Kit

Materials and procedure Step-by-Step Guide for Photocatalysis High-Throughput Reaction Screening Kit

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

脂肪酶活性测定实验方案

酸性磷酸酶的酶促测定（EC 3.1.3.2）

Performing a Separation with Sephadex^®

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit

Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending

GenElute™ 哺乳动物基因组 DNA 微量提取试剂盒实验方案

GenElute 哺乳动物基因组 DNA 纯化方案阐述了从多种哺乳动物来源中分离纯高分子量 DNA 的方法。

Heavy Metals in Textiles

Sample preparation and determination of heavy metals in textiles.

抑肽酶酶活测定

目标：建立抑肽酶酶活测定的标准化操作步骤。

Assay Procedure for Invertase

The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.

酵母生长实验方案

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products on Sigma.com.

Amplification of Genomic DNA using REDTaq DNA Polymerase

Method for PCR using genomic DNA or other complex templates

Peptide Quick Tips

Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

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