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SEQR – SeqPlex RNA扩增试剂盒实验方案
SeqPlex RNA扩增试剂盒可在进入常用深度测序平台工作流程之前为总RNA或分离后的mRNA提供一种扩增方法,
食品酶法分析
用于食品分析的酶法具有高度特异性,与其他方法相比,特别是从样品制备的角度来看,可以节省大量时间和成本。我们提供各种方便的试剂盒和试剂,用于快速可靠的酶食品分析。
OverExpress™ Chemically Competent Cells
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
Cell Proliferation ELISA, BrdU (colorimetric) Protocol & Troubleshooting
Cell Proliferation ELISA, BrdU (colorimetric) Protocol & Troubleshooting
Purification of Protein A-Tagged Proteins
This page shows how to purify protein A-tagged proteins using GE Healthcare products.
Elemental Impurities - Certified Reference Materials for ICH Q3D, USP 232, USP 2232, and Ph.Eur. 5.20
Elemental impurities in drug products pose a risk to patient health due to toxicological effects, and their levels should be controlled within acceptable limits. Find analytical methods and materials for impurity assessment.
RAFT聚合反应操作步骤
RAFT(可逆加成-断裂链转移)是一种活性自由基聚合反应,在有适合的连转移(RAFT)试剂存在下发生的非传统的取代单体自由基聚合反应。
Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra products are magnetic beads designed for high capacity small-scale purification/screening of monoclonal and polyclonal antibodies from various species.
Recombinant eSpCas9 Protein for RNP-Based Genome Editing
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.
How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System
Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.
Using Duolink™ in Multiwell Plates
When using the Duolink reagents to stain cells grown in multiwell plates, a few modifications to the Duolink protocol, optimized for staining samples deposited on glass slides, have to be made in order to suit the multiwell format. In particular
Coronavirus qPCR Design Case Study & Related Products to Support SARS-CoV-2 Research
Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
Analytical Method: Lead in Waste Water with High Concentrations of Calcium
Photometric determination with PAR subsequent to separation of lead with Sodium diethyldithiocarbaminate
SygRNA®合成CRISPR向导RNA
Sigma-Aldrich® sgRNA、crRNA和tracrRNA统一采用HPLC纯化,包括定制设计产品,均可确保性能。
Analytical Method: Silicon (total) in iron oxide powder
Photometric determination using the Silicon molybdenum blue method subsequent to fusion melting
Hot Start dNTP protocol to reduce non-specific amplification
Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
以酪蛋白为底物酶法测定蛋白酶
建立以酪蛋白为底物的蛋白酶的标准酶法测定方法。
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.
Performing a Separation with HiTrap® IgM Purifcation HP
How to perform a separation with the HiTrap IgM Purifcation HP from GE Healthcare which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.
NBT实验方案和故障排除
存在有多种可与BM Purple(或通常的NBT/BCIP)组合的复染剂,包括FastGreen FCF和Nuclear Fast Red。
Dual-Column GC Analysis of Organochlorine Pesticides on SLB®-5ms and SLB®-35ms
The primary column is used to determine presence of the target analytes in the sample; the secondary column of a different selectivity confirms whether a positive match.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.
Enzymatic Assay of Glucose Oxidase
Enzymatic Assay of Glucose Oxidase
Reduce Matrix Background and Improve Overall Analyte Recovery for the Analysis of Pesticides in Beef Kidney
Supel QuE Z-Sep+ was successfully demonstrated for cleanup of beef kidney matrix, providing reduced background and less interference in the GC-MS analysis of the target pesticides
qPCR效率测定实验方案
QPCR 条件的优化对于制定稳健的检测方案非常重要。两种主要优化方法是优化引物浓度和/或退火温度。
变溶菌素的酶促检测
该操作步骤可用于变溶菌素产物。
Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
Performing a Small-Scale Expression Screening of Histidine-Tagged Membrane Proteins from E. coli Lysates
This page shows how to perform a small-scale expression screening of histidine-tagged membrane proteins from E. coli lysates with His MultiTrap filter plates from GE Healthcare.
Step-by-Step Guide for KitAlysis™ High-Throughput Metal Scavenger Screening Kit
Materials included and protocol for your KITALYSIS-SCAV-2PK High-Throughput Screening Kit
Enzymatic Method for Determining D-Sorbitol (D-Sorbitol Assay)
Assay protocol for the colorimetric detection of D-Sorbitol in samples using the D-Sorbitol assay kit.
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