Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This can hinder the researcher’s ability to perform downstream analysis. The following protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.
Required Products
Additional Materials Required
Extraction of DNA from a Whole Blood
The GenElute Blood Genomic DNA Kit (Product No. NA2000) is recommended for this procedure.
Legend: Products were amplified using the GenomePlex Whole Genome Amplification Kit (Product No. WGA1), Supplier A’s kit and Supplier Q’s kit. Products were resolved on a 1.5% agarose gel. 5 µL of amplified product was added to each well. The products amplified using GenomePlex technology were of a smaller molecular weight as shown on the gel when compared to Supplier A and Q.
This is due to the random fragmentation of genomic DNA before amplification. Our amplified products are specific and there is no amplicon visible in the negative control (lane 2) indicating that only the desired genomic DNA is amplified. Both Suppliers A and Q yield a nonspecific signal in the negative control which is equal in size and intensity to the signal for the suppliers’ positive control.
Legend: Products were amplified using the GenomePlex Whole Genome Amplification Kit (Product No. NA2000). 10 ng of genomic DNA was amplified using the GenomePlex WGA Kit (Product No . WGA1) followed by purification using the GenElute PCR Clean-up Kit (Product No. NA1020). SNP genotyping analysis was performed on non-amplified DNA and GenomePlex amplified DNA. GenomePlex WGA DNA genotyping provided the same accuracy and quality of scores to non-amplified DNA, indicating that the amplification process did not alter the original genomic sequence.
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