Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases. However, the concentration of urine proteins is often below the detection limit of clinical tests. Amicon® Ultra-4 mL 10 kDa MWCO devices can be used to concentrate urine samples prior to clinical laboratory analyses.
Enrichment of free immunoglobulin light chains (Bence-Jones proteins) in urine samples using Amicon® Ultra-4 mL 10 kDa MWCO filters.
Results of electrophoretic resolution of free immunoglobulin light chains (Bence-Jones proteins) in urine samples concentrated using Centricon® (C) and Amicon® Ultra (A) centrifugal concentration devices. The dark bands at ~25 kDa in each lane represent these proteins.
Research using Amicon® Ultra devices for urine concentration in this protocol was conducted by Mark Merchant, Ph.D. at Helena Laboratories, Beaumont, TX.
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