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安全信息

MSANGERV

Sigma-Aldrich

桑格全基因组 慢病毒 CRISPR 文库

Mouse, Virus Format

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质量水平

包装

pkg of 10 μL (384-well plate)

浓度

1x106  VP/ml (via p24 assay)

应用

CRISPR

运输

dry ice

储存温度

−70°C

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一般描述

两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了第一个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术,Sanger CRISPR库将使您的实验室走在竞争的最前沿,以实现下一个重大发现。

应用

功能基因组学/筛选/靶标验证

特点和优势

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

包装

384 well plates

组分

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


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外形

10μl of lentivirus at ≥106 particles/ml in ~120×384 well plates

其他说明

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

推荐产品

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

分析证书(COA)

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Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

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部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al
Scientific Reports, 7 (2017)

商品

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

实验方案

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

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