Quality Level
packaging
pkg of 10 μL (384-well plate)
concentration
1x106 VP/ml (via p24 assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
General description
两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了第一个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术，Sanger CRISPR库将使您的实验室走在竞争的最前沿，以实现下一个重大发现。
Application
功能基因组学/筛选/靶标验证
Features and Benefits
- Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
- Simplify the workflow with puromycin selection
- Illuminate CRISPR-expressing cells with BFP
Additional Features
- Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
- Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
- Collaborative: Real-time, library validation continues
For detailed information on the Sanger library, click here
Packaging
384 well plates
Physical form
10μl of lentivirus at ≥106 particles/ml in ~120×384 well plates
Other Notes
Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones
Request a Quote or More Information
Request a Quote or More Information
This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.
Legal Information
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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实验方案
了解桑格测序步骤或链终止法、DNA 测序的工作原理以及如何在研究中准确读取桑格测序结果。
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
商品
全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在，每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量，可确保尽可能特异性的筛选，同时控制时间和成本。
获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.