{"lastModifiedDate":1645984321079,"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"publishedDate":1645988154450,"lastModifiedDate":1645984321079,"keywords":["Analytical chemistry","Small molecule HPLC","Protocol"],"title":"HPLC Analysis of Vitamins B1 B6 B12","seoSchemas":[{"itemListElement":[{"position":1,"name":"Small Molecule HPLC","item":"%%HOMEPAGE_URL%%/CN/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc","@type":"ListItem"},{"position":2,"name":"HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis^® Express RP-Amide","@type":"ListItem"}],"@context":"https://schema.org","@type":"BreadcrumbList"}],"description":"HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide","pageType":"Protocol","ogImageMetaInfo":null,"translationMethod":null,"canonicalUrl":null},"language":"en","title":"HPLC Analysis of Vitamins B1 B6 B12","cssClassNames":"page basicpage","templateName":"technical-article---protocol-page-template","designPath":"/libs/settings/wcm/designs/default","hasAnnotations":false,":path":"/CN/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/hplc-analysis-of-vitamins-b1-b6-b12",":items":{"root":{"columnCount":12,"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12",":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-567a908a9b","allowedComponents":{"applicable":false,"components":[]},":items":{"tagbasedbreadcrumb":{"items":[{"url":"/CN/en","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/CN/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc","title":"Small Molecule HPLC","titleEng":"Small Molecule HPLC","active":false,"nonClickable":false},{"url":"/CN/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/hplc-analysis-of-vitamins-b1-b6-b12","title":"HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis^® Express RP-Amide","titleEng":"HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis^® Express RP-Amide","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"text":"HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis^® Express RP-Amide","linkURL":null,"openNewTab":false,"type":"h1",":type":"cms-commons/components/content/title"}},":itemsOrder":["tagbasedbreadcrumb","title"],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-b9ee85d341","allowedComponents":{"applicable":false,"components":[]},":items":{},":itemsOrder":[],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-0ac85f47a0","allowedComponents":{"applicable":false,"components":[]},":items":{"topcontainer":{"columnClassNames":{"image":"aem-GridColumn aem-GridColumn--default--8 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","table_1566999904":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-369e30f296","allowedComponents":{"applicable":false,"components":[]},":items":{"image":{"anchorId":null,"title":"HPLC Analysis of Vitamins B1, B6 and B12","src":"/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/protocols/analytical-chemistry/small-molecule-hplc/hplc-analysis-vitamin-b.jpg","uuid":"e3df30cc-75d3-4e42-bc5d-9b8a3c99e775","link":"","alt":"HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide","widths":[],"externalUrl":false,"captionTitle":"Figure 1.",":type":"cms-commons/components/content/image","caption":"HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide","displayPopupTitle":false},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Column	Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50773-U)
Column Temp.	30 °C
Mobile Phase	[A] 0.02 M Ammonium Formate, pH = 3.0; [B] Acetonitrile
Gradient	0-50% B in 1 min. Hold at 50% B until 1.5 min
Flow Rate	1.5 mL/min
Pressure	1639 psi (113 bar)
Sample	Dissolved in 50/50 water/ACN
Injection	1 µL
Detector	UV, 276 nm

\r\n",":type":"cms-commons/components/content/table"},"table_1566999904":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Categories	Analytical Chromatography, Vitamins and Natural products
Featured Industry	Vitamins, Nutraceuticals, and Natural Products
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for HPLC

\r\n",":type":"cms-commons/components/content/table"}},":itemsOrder":["image","table","table_1566999904"],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""},"dynamicproducttable":{"anchorId":null,"productsArray":["1.00029 Supelco (MM)","1.00030 Supelco (MM)","1.06007 Supelco (MM)","1.06035 Supelco (MM)","1.15333 Supelco (MM)","1.11727 Supelco (MM)","5.33001 Supelco (MM)","5.33002 Supelco (MM)","69372 Millipore (SIAL)","70221 Supelco (SIAL)","73594 Supelco (SIAL)","5.43841 Supelco (MM)","70221 Supelco (SIAL)","34998 Sigma-Aldrich (SIGALD)","V-014 Supelco (CERILLIAN)","V-019 Supelco (CERILLIAN)","50773-U Supelco (SUPELCO)"],"isShowImage":false,"maxRows":20,":type":"cms-commons/components/content/dynamicproducttable","tableTitle":"Materials","products":[{"productKey":"100029","brandKey":"MM"},{"productKey":"100030","brandKey":"MM"},{"productKey":"106007","brandKey":"MM"},{"productKey":"106035","brandKey":"MM"},{"productKey":"115333","brandKey":"MM"},{"productKey":"111727","brandKey":"MM"},{"productKey":"533001","brandKey":"MM"},{"productKey":"533002","brandKey":"MM"},{"productKey":"69372","brandKey":"SIAL"},{"productKey":"70221","brandKey":"SIAL"},{"productKey":"73594","brandKey":"SIAL"},{"productKey":"543841","brandKey":"MM"},{"productKey":"70221","brandKey":"SIAL"},{"productKey":"34998","brandKey":"SIGALD"},{"productKey":"V014","brandKey":"CERILLIAN"},{"productKey":"V019","brandKey":"CERILLIAN"},{"productKey":"50773U","brandKey":"SUPELCO"}]},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-0a5111ce0f","allowedComponents":{"applicable":false,"components":[]},":items":{},":itemsOrder":[],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-969e10c178","allowedComponents":{"applicable":false,"components":[]},":items":{},":itemsOrder":[],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""}},":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""},"rightrailresponsivecontainer":{"columnClassNames":{"relatedcontentlinks_":"aem-GridColumn aem-GridColumn--default--3","cq:responsive":"aem-GridColumn aem-GridColumn--default--3","relatedcontentlinks__793030730":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"id":"container-729087d252","allowedComponents":{"applicable":false,"components":[]},":items":{"relatedcontentlinks_":{"contentType":"sigma-aldrich:content-type/page-type/protocol","linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"/CN/en/search/facet-search?focus=sitecontent&&term=facet-search&facet=facet_content_type%3AProtocol&facet=facet_applications%3ASmall+molecule+HPLC","title":"Related Articles","tags":["sigma-aldrich:applications/analytical-chemistry/small-molecule-hplc"],"ctaTitle":null,"categories":[{"categoryTitle":"Analysis of Alkylphenols Using New Internal Standards","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/analysis-of-alkylphenols","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Neonicotinoids","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/neonicotinoids-bee-pesticides","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Syringe Care","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/gas-chromatography/general-syringe-care","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Analysis of Active Cannabis Compounds","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/quechers/analysis-of-active-cannabis-compounds","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Neonicotinoids Ban: Impact on Plant Material","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/quechers/banned-other-neonicotinoids-plant-material","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/analysis-of-25-hydroxyvitamin-d2","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Analysis of 17 Cannabinoids in Hemp and Cannabis","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/analysis-of-cannabinoids","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false},{"categoryTitle":"Analysis of Polar Compounds with Ion Pair Reagents","categoryUrl":"/CN/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/analysis-of-difficult","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"},"relatedcontentlinks__793030730":{"linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"","title":"Related Product Categories","tags":null,"ctaTitle":null,"categories":[{"categoryTitle":"Controlled Substances & Illicit Drug Standards","categoryUrl":"/CN/en/products/analytical-chemistry/reference-materials/pharmaceuticals-illicit-drugs-and-diagnostic-biomarkers","thumbnail":"/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/analytical-chemistry/certified-reference-materials-for-pharmaceuticals.jpg","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"}},":itemsOrder":["relatedcontentlinks_","relatedcontentlinks__793030730"],":type":"cms-commons/components/content/container","desktopSpacing":{},"mobileSpacing":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":""},"footer":{"id":"footer-57681991bd","richText":false,":type":"cms-commons/components/content/footer","dataLayer":{"footer-57681991bd":{"@type":"cms-commons/components/content/footer","repo:modifyDate":"2022-02-27T17:52:01Z","xdm:text":""}}}},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":type":"wcm/foundation/components/responsivegrid"}},":itemsOrder":["root"],":type":"cms-commons/components/structure/page",":hierarchyType":"page"}

HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis® Express RP-Amide

Materials

HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis^® Express RP-Amide