Anti-IRS1 Antibody, clone AW58, rabbit monoclonal

Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.

Mr ~145-165kDa

Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1

Anti-IRS1 Antibody, clone AW58 detects level of IRS1 & has been published & validated for use in IP & WB.

Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.

Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

IRS1

Cultured supernantant in 0.05% sodium azide

1 year at -20°C from date of shipment

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts

Replaces: 05-784

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