Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463, rabbit monoclonal

17kDa

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine

Peptide containing the sequence RK[pS]TG. in which pS corresponds to phospho-serine at position 10 of human histone H3

Anti-phospho-Histone H3 (Ser10) Antibody, clone MC463 is a rabbit monoclonal antibody for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p, is specificity verified using DB & Mplex, published in peer reviewed journals & validated in ChIP, IF, WB, ICC, ChIP-seq.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis:
A 1:2,000-1:4,000 dilution of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid (lane 1) vs. untreated cells (lane 2) (Figure A).

Beadlyte Histone-Peptide Specificity Assay:
A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.

Immunofluorescence:
Representative image from a previous lot.
Positive chromosome immunostaining for mitotic HeLa cells and A431 cells.


Chromatin Immunoprecipitation:
A previous lot was shown to be suitable for ChIP by an independent laboratory.

Broad species cross-reactivity is expected

Histone H3 phosphorylated at Serine 10

Cultured supernantant in 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts

routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells

Replaces: 05-817

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