05-1427
Anti-ARF1 Antibody, clone 3F1
clone 3F1, from mouse
Synonym(s):
ADP-ribosylation factor 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F1, monoclonal
Application:
ELISA, IHC, WB
Citations:
-
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F1, monoclonal
species reactivity
rat, human, mouse
technique(s)
ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ARF1(375)
mouse ... Arf1(11840)
General description
ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
~21 kDa
Immunogen
Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
Application
Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
Biochem/physiol Actions
Recognizes human ARF1
Physical form
Format: Purified
Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide
Analysis Note
Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
Other Notes
Replaces: MAB3779
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-1427 | 04053252277665 |