05-504
Anti-IQGAP1 Antibody, clone AF4
clone AF4, Upstate®, from mouse
Synonym(s):
Anti-HUMORFA01, Anti-SAR1, Anti-p195
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AF4, monoclonal
Application:
IP, WB
Citations:
12
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
AF4, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IQGAP1(8826)
General description
185kDa
Immunogen
A Glu-Glu tagged C-terminal fragment corresponding to amino acids 863-1657 of human IQGAP1, purified from baculovirus infected insect cells
Application
Detect IQGAP1 using this Anti-IQGAP1 Antibody, clone AF4 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
IQGAP1, not IQGAP2
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein G Chromatography
Preparation Note
2 years at -20°C
Analysis Note
routinely evaluated by immunoblot on HepG2 RIPA cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
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Dynamics of ligand-induced, Rac1-dependent anchoring of cadherins to the actin cytoskeleton.
Lambert, et al.
The Journal of Cell Biology, 157, 469-479 (2018)
M J Hart et al.
The EMBO journal, 15(12), 2997-3005 (1996-06-17)
Proteins that associate with the GTP-bound forms of the Ras superfamily of proteins are potential effector targets for these molecular switches. A 195 kDa protein was purified from cell lysates by affinity chromatography on immobilized cdc42Hs-GTP and a corresponding cDNA
S Kuroda et al.
Biochemical and biophysical research communications, 262(1), 1-6 (1999-08-17)
Cell-cell adhesion is a dynamic process in various cellular and developmental situations. Cadherins, well-known Ca(2+)-dependent adhesion molecules, are thought to play a major role in the regulation of cell-cell adhesion. However, the molecular mechanism underlying the rearrangement of cadherin-mediated cell-cell
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-504 | 04053252621260 |