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06-503-I

Anti-Caspase 1 Antibody

Name: Anti-Caspase 1 Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, WB

Citations:

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Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

NCBI accession no.

NP_001214

UniProt accession no.

P29466

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP1(834)

Description

General description

~45 kDa observed. Five isoforms between 10-45 kDa are known to exist. This antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.

Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an enzyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.

Immunogen

KLH-conjugated linear peptide corresponding to the p20 subunit of human Caspase 1.

Epitope: p20 subunit

Application

Anti-Caspase 1 Antibody is a highly specific rabbit polyclonal antibody, that targets Caspase 1 & has been tested in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Caspase 1 in human spleen tissue. Additionally, a 1:4,000 dilution from a representative lot detected Caspase 1 in human colon tissue.

Research Sub Category
Caspases

Biochem/physiol Actions

Other homologies: Mouse, rat, canine, porcine and feline. (92% sequence homology).

This antibody recognizes the p20 subunit of Caspase 1. This antibody may cross-react with Caspase 5 due to 92% sequence homology. Additionally, this antibody recognizes the P45 proenzyme (~45 kDa) and the p20 subunit (~20 kDa) of the reduced, human Caspase 1 isoforms.

Analysis Note

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected Caspase 1 in 10 µg THP-1 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 06-503

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c