Anti-GLUT-4 Antibody, C-terminus

Solute carrier family 2, facilitated glucose transporter member 4 (UniProt: P14672; also known as Glucose transporter type 4, insulin-responsive, GLUT-4) is encoded by the SLC2A4 (also known as GLUT4) gene (Gene ID: 6517) in human. Glucose transport in mammalian cells depends upon membrane-associated carrier proteins. Mammalian cells possess two types of glucose carriers (a) the Na+-glucose co-transporter and the (b) facilitative glucose transporter. The Na+-glucose co-transporter transports glucose against its concentration gradient by coupling its uptake with the uptake of Na+. It is largely expressed in epithelial cells of the small intestine and kidney. The facilitative glucose carriers of the GLUT family accelerate the transport of glucose down its concentration gradient by facilitative diffusion. Members of the GLUT family are expressed in a tissue- and cell-specific manner and exhibit distinct kinetic and regulatory properties that reflect their specific functional roles. GLUT4 is a multi-pass membrane protein with 6 extracellular domains, 12 transmembrane domains, and 7 cytoplasmic domains. It is expressed in insulin sensitive tissues, such as muscle and adipose tissue and its levels change in response to insulin. It is responsible for increased glucose disposal in these tissues in the postprandial state and is vital in whole-body glucose homeostasis. In the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. However, upon insulin stimulation, it translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell. GLUT-4 contains a dileucine internalization motif (aa 489-490) that is critical for its intracellular sequestration. Mutations in SLC2A4 gene are known to cause non-insulin-dependent diabetes mellitus, a multifactorial disorder of glucose homeostasis. GLUT4 function as well as its transcriptional regulation is highly conserved throughout the vertebrate species.

~58 kDa observed

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human GLUT-4.

Anti-GLUT-4, C-terminus, Cat. No. 07-1404, is a rabbit polyclonal antibody that detects GLUT-4 and is tested for use in Western Blotting and Immunohistochemistry (Paraffin).

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Tested Applications

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected GLUT-4 in lysates from Human umbilical vein endothelial cells (HUVEC) and HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected GLUT-4 in human heart tissue sections and a 1:100 dilution from a representative lot detected GLUT-4 in human skeletal muscle and, human salivary gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This rabbit polyclonal antibody specifically detects GLUT-4. It targets an epitope within 13 amino acids from the C-terminal cytoplasmic domain.

Affinity purified

Purified rabbit polyclonal antibody in PBS with 0.1% sodium azide.

Recommended storage: +2°C to +8°C.

Control
NIH3T3 cell lysate

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected GLUT-4 in NIH/3T3 cell lysate.

Replaces: AB1346

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.