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13-173

MBP, recombinant

Name: MBP, recombinant
Brand: MM

Synonym(s):

myelin basic protein

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.41

eCl@ss:

32160405

Form:

liquid

Biological source:

bovine

Mol wt:

M_w ~47 kDa

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Properties

biological source

bovine

Quality Level

100

form

liquid

mol wt

M_w ~47 kDa

manufacturer/tradename

Upstate^®

concentration

1 mg/mL

technique(s)

activity assay: suitable

NCBI accession no.

NM_001025081.1, NM_001025090.1, NM_001025092.1, NM_001025094.1, NM_001025098.1, NM_001025100.1, NM_001025101.1, NM_002385.2

UniProt accession no.

P02686

shipped in

dry ice

Description

General description

Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.

Physical form

500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.

ProBond Resin chromatography

Preparation Note

Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Routinely evaluated by kinase assay

Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.

Other Notes

Purity information is lot specific, please consult the certificate of analysis

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c