16-316
Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate
Upstate®, from mouse, peroxidase conjugate
Synonym(s):
phosphotyrosine
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
4G10®, monoclonal, PY20, monoclonal
Application:
ELISA, WB
Citations:
8
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody product type
primary antibodies
clone
4G10®, monoclonal, PY20, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG2b
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone.
Varies
Application
Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.
Physical form
100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10 (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing preservative and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.
Analysis Note
Routinely evaluated by western blot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
4G10 is a registered trademark of Upstate Group, Inc.
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
Wei Tian et al.
BMC biochemistry, 13, 22-22 (2012-10-25)
Phosphatase of regenerating liver-3 (PRL-3 or PTP4A3) has been implicated in controlling cancer cell proliferation, motility, metastasis, and angiogenesis. Deregulated expression of PRL-3 is highly correlated with cancer progression and predicts poor survival. Although PRL-3 was categorized as a tyrosine
Identification of a novel EphB4 phosphodegron regulated by the autocrine IGFII/IRA axis in malignant mesothelioma.
Scalia, et al.
Oncogene, 38, 5987-6001 (2021)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 16-316 | 04053252729423 |
| Z261580-1EA | 04061832530970 |
| Z261599-1EA | 04061832530987 |
| Z261610-1EA | 04061832530994 |
| Z261629-1EA | 04061832531007 |