386032
Anti-Hsp70 Mouse mAb (C92F3A-5)
liquid, clone C92F3A-5, Calbiochem®
Synonym(s):
Anti-Heat Shock Protein 70
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
C92F3A-5, monoclonal
Species reactivity:
-
Application:
—
Citations:
5
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
C92F3A-5, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Flow Cytometry
Frozen Sections (5 µg/mL)
Immunoblotting (1 µg/mL)
Immunocytochemistry (5 µg/mL)
Immunoprecipitation
Paraffin Sections (5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSPA1A(3303)
General description
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
Immunogen
Human
Hsp70 from HeLa cells
Application
ELISA (see comments)
Flow Cytometry (see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 µg/ml)
Flow Cytometry (see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% glycerol, pH 7.2.
Preparation Note
Following iniital thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
L929 cells, Human colon cancer tissue
L929 cells, Human colon cancer tissue
Other Notes
Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Aigang Lu et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 22(2), 183-195 (2002-02-02)
Estradiol reduces brain injury from many diseases, including stroke and trauma. To investigate the molecular mechanisms of this protection, the effects of 17-beta-estradiol on heat shock protein (HSP) expression were studied in normal male and female rats and in male
Anne K Voss et al.
The EMBO journal, 25(15), 3652-3663 (2006-07-22)
The mechanisms regulating the size of the cerebral cortex are poorly understood. Here, we demonstrate that the Rap1 guanine nucleotide exchange factor, C3G (Grf2, Rapgef1), controls the size of the cerebral precursor population. Mice lacking C3G show overproliferation of the
Kamlesh K Bhopale et al.
Alcoholism, clinical and experimental research, 41(10), 1675-1685 (2017-08-10)
Chronic alcohol abuse, a major risk factor for such diseases as hepatitis and cirrhosis, impairs hepatic alcohol dehydrogenase (ADH; key ethanol [EtOH]-metabolizing enzyme). Therefore, differentially altered hepatic and plasma proteomes were identified in chronic EtOH feeding model of hepatic ADH-deficient
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 386032-50UG | 04055977189889 |