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AB1526P

Sigma-Aldrich

Anti-Nerve Growth Factor-β Antibody, pain

Chemicon®, from rabbit

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Synonym(s):
NGFbeta
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

chicken, human, mouse, rat

should not react with

bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_002506.2

UniProt accession no.

P01138

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NGF(4803)

Specificity

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human BDNF, NT3 and NT4 by ELISA.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Application

Immunohistochemistry: 1-2 μg/mL (see suggested protocol).

Immunoblotting: 1-2 μg/mL

Bioassays (Inhibition of biological activity in vitro): 1-2 μg/mL

in vivo animal studies: use neat

ELISA: 1-2 μg/mL

Optimal working dilutions must be determined by the end user.

BIOLOGICAL ACTIVITY:

This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor-β Antibody, pain is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.

Linkage

Replaces: 04-1119

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute to desired volume with sterile distilled water. Suggested concentration 0.5-1.0 mg/mL.

Storage and Stability

Maintain at lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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