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Merck
CN

AB1585

Anti-Dopamine β Hydroxylase Antibody

serum, Chemicon®

Synonym(s):

DBH, Dopamine beta-monooxygenase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, RIA, WB
Citations:
41
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

bovine, guinea pig, rat, human, feline, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DBH(1621)

General description

69 kDa

Immunogen

Native enzyme from bovine adrenal glands.

Application

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody validated for use in IH, FUNC, RIA & WB.
Immunohistochemistry: 1:2,000 on rabbit and guinea pig using an ABC detection method.

Western blot

RIA

Inhibitory studies in vivo and in vitro.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Biochem/physiol Actions

Recognizes Dopamine beta hydroxylase. AB1585 reacts with a single band on Western blots of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with enzyme.

Physical form

Rabbit Serum. Lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.
Unpurified

Preparation Note

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. Glycerol can be added 1:1 for additional stability.

Analysis Note

Control
Brain (Striatum/Substantia Nigra)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB145

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The toxicity of tumor necrosis factor-alpha upon cholinergic neurons within the nucleus basalis and the role of norepinephrine in the regulation of inflammation: implications for Alzheimer's disease.
Wenk, GL; McGann, K; Hauss-Wegrzyniak, B; Rosi, S
Neuroscience null
Sex and species differences in tyrosine hydroxylase-synthesizing cells of the rodent olfactory extended amygdala.
Katharine V Northcutt, Zuoxin Wang, Joseph S Lonstein
The Journal of Comparative Neurology null
Yan Lin et al.
Pharmacology, biochemistry, and behavior, 100(2), 330-339 (2011-09-07)
Several lines of research have now suggested the controversial hypothesis that the central noradrenergic system acts to exacerbate depression as opposed to having an antidepressant function. If correct, lesions of this system should increase resistance to depression, which has been



Global Trade Item Number

SKUGTIN
AB158504053252732089