AB2083
Anti-CD44v7 Antibody, exon v7
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, FACS, ICC, IHC, IP, WB
Citations:
7
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, bovine, rabbit, rat, canine, pig
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CD44(960)
Immunogen
Epitope: exon v7
Synthetic peptide of human CD44v7
Application
Detect CD44v7 using this Anti-CD44v7 Antibody, exon v7 validated for use in ELISA, FC, IP, WB, IC, IH(P).
ELISA: 1:1,000-1:2,000
Flow Cytometry: 1:500-1:1,000
Immunofluorescence: 1:100-1:200
Immunohistostaining of paraffin embedded tissue: 1:150-1:250
Immunoprecipitation: 1:100-1:200
Western Blotting: 1:5,000-1:10,000
Optimal working dilutions must be determined by the end user.
Flow Cytometry: 1:500-1:1,000
Immunofluorescence: 1:100-1:200
Immunohistostaining of paraffin embedded tissue: 1:150-1:250
Immunoprecipitation: 1:100-1:200
Western Blotting: 1:5,000-1:10,000
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
CD44v7 (Approximant 200-250 kDa)
Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. For example, epithelial cells contain exons 12-14 inserted into the CD44s transcripts. This isoform has been designated as CD44E. Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane. It has been suggested that expression of CD44 variants with the additional exon 11 (so-called v7) is responsible for tumor progression and metastasis. In fact, CD44v7 expression has been used as an indicator of metastasis
Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. For example, epithelial cells contain exons 12-14 inserted into the CD44s transcripts. This isoform has been designated as CD44E. Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane. It has been suggested that expression of CD44 variants with the additional exon 11 (so-called v7) is responsible for tumor progression and metastasis. In fact, CD44v7 expression has been used as an indicator of metastasis
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl with 0.01% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1123
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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CD44 differentially activates mouse NK T cells and conventional T cells.
Jonathan Larkin, Gourapura J Renukaradhya, Venkataraman Sriram, Wenjun Du et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Surface protein expression and messenger RNA-splicing analysis of CD44 in uterine cervical cancer and normal cervical epithelium.
Dall, P; Heider, KH; Hekele, A; von Minckwitz, G; Kaufmann, M; Ponta, H; Herrlich, P
Cancer Research null
R Arch et al.
Science (New York, N.Y.), 257(5070), 682-685 (1992-07-31)
A variant of the glycoprotein CD44 (CD44v) that shares sequences with variants causally involved in metastasis formation is transiently expressed on B and T lymphocytes and macrophages after antigenic stimulation and in the postnatal period. Antibodies to the variant hinder
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB2083 | 04053252322921 |