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Merck
CN

AB3530P

Anti-Epithelial Sodium Channel-α Antibody

Chemicon®, from rabbit

Synonym(s):

ENaC-alpha

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
5
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SCNN1A(6337)

General description

83-90kDa

Immunogen

A 20 amino acid peptide sequence near the N-terminus of rat ENaC alpha (Linguela et al. 1993; Canessa et al.

1993; Snyder et al. 1994; Waldmann et al. 1995; Kreutz et al. 1997; Voilley et al.

1994; McDonald et al. 1994; Ludwig et al. 1998; Garty & Palmer 1997).

Application

Anti-Epithelial Sodium Channel-α Antibody is an antibody against Epithelial Sodium Channel-α for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Neuroinflammation & Pain
Western blot: 1-10 μg/mL using ECL and rat brain membrane preparations, see membrane preparation protocol under Technical Support\General Technical Info\General Methods at www.chemicon.com.

83-90kDa band is expected.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes rat Epithelial Sodium Channel alpha (ENaC alpha). The immunogen shows no significant sequence homology with other ENaC subunits.

Physical form

Affinity Purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA and 0.1% sodium azide.
ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Epithelial structures

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Estrogen increases ENaC activity via PKC? signaling in renal cortical collecting duct cells.
Yusef, YR; Thomas, W; Harvey, BJ
Physiological Reports null
Expression of water and ion transporters in tracheal aspirates from neonates with respiratory distress.
Li Y, Marcoux MO, Gineste M, Vanpee M, Zelenina M, Casper C
Acta Paediatrica (Oslo, Norway : 1992) null
Unmasking a sustained negative effect of SGLT2 inhibition on body fluid volume in the rat.
Masuda, et al.
American Journal of Physiology: Renal Physiology, 315, F653-F664 (2020)



Global Trade Item Number

SKUGTIN
AB3530P04053252336423