AB3832
Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific
Chemicon®, from rabbit
Synonym(s):
Anti-EBP50, Anti-NHERF, Anti-NHERF-1, Anti-NHERF1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
monkey, human, mouse, rat
Application:
IHC, WB
Citations:
1
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
monkey, human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr567)
Gene Information
human ... SLC9A3R1(9368)
General description
75KDa Moesin, and ~80kDa Ezrin & Radixin
Immunogen
Epitope: phospho-specific
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin
Application
Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.
Immunohistochemistry (paraffin): 1:50
Optimal working dilutions must be determined by the end user.
Immunohistochemistry (paraffin): 1:50
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.
Physical form
ImmunoAffinity Purified
Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.
Preparation Note
Maintain at -20°C for 12 months, do not aliquot.
Analysis Note
Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Minji Kim et al.
Journal of cell science, 128(23), 4317-4327 (2015-10-21)
Tubulogenesis is fundamental to the development of many epithelial organs. Although lumen formation in cysts has received considerable attention, less is known about lumenogenesis in tubes. Here, we utilized tubulogenesis induced by hepatocyte growth factor (HGF) in MDCK cells, which
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB3832 | 04053252279188 |