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Merck
CN

AB5447

Anti-Tropomyosin Antibody, 5NM1 and 5NM2

Chemicon®, from sheep

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, WB
Citations:
13
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biological source

sheep

Quality Level

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TPM3(7170)

Immunogen

Epitope: 5NM1 and 5NM2
Synthetic peptide corresponding to the 9d exon from the gamma gene of tropomyosin.

Application

Detect Tropomyosin using this Anti-Tropomyosin Antibody, 5NM1 & 5NM2 validated for use in WB, IC, IH.
Research Category
Metabolism
Research Sub Category
Muscle Physiology
Western Blot: 1:100-1:500. Antibody reacts with a band at 29-30kDa.

Immunocytochemistry: 1:100-1:500

Immunohistochemistry: 1:100-1:500

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Tropomyosin 5NM1 and 5NM2.

Physical form

Affinity purified immunoglobulin in PBS (liquid).

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The molecular composition of neuronal microfilaments is spatially and temporally regulated.
Weinberger, R, et al.
The Journal of Neuroscience, 16, 238-252 (1996)
Functional effects of mutations in the tropomyosin-binding sites of tropomodulin1 and tropomodulin3.
Lewis, RA; Yamashiro, S; Gokhin, DS; Fowler, VM
Cytoskeleton (Hoboken, N.J.) null
Staffan Grenklo et al.
European journal of cell biology, 87(11), 905-920 (2008-09-03)
Tropomyosin is a coiled-coil alpha-helical protein, which self-associates in a head-to-tail fashion along polymers of actin to produce thin filaments. Mammalian non-muscle cells express a large number of tropomyosin isoforms, which are differentially regulated during embryogenesis and associated with specialized



Global Trade Item Number

SKUGTIN
AB544704053252582332