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ABS194

Anti-phospho PDHE1-A type I (Ser300) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
ICC, WB
Citations:
1
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biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NP_000275

UniProt accession no.

P08559

shipped in

wet ice

target post-translational modification

phosphorylation (pSer300)

Gene Information

human ... PDHA1(5160)

General description

In many organisms, the pyruvate dehydrogenase complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic, energy-generating pathways, in addition to serving as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
~43 kDa observed

Immunogen

Epitope: Phosphorylated Ser300
KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser300.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PDHE1-A type I in dichloroacetate untreated and treated HEK 293 cells.
This Anti-phospho PDHE1-A type I (Ser300) Antibody is validated for use in WB, IC for the detection of phospho PDHE1-A type I (Ser300).

Biochem/physiol Actions

This antibody recognizes PDHE1-A type I phosphorylated at Ser300.

Analysis Note

Control
Dichloroacetate untreated and treated HEK 293 cell lysates
Evaluated by Western Blot in dichloroacetate untreated and treated HEK 293 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDHE1-A type I on 10 µg of dichloroacetate untreated and treated HEK 293 cell lysates.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hao Shi et al.
Molecular metabolism, 11, 160-177 (2018-03-12)
Given that cellular O-GlcNAcylation levels are thought to be real-time measures of cellular nutrient status and dysregulated O-GlcNAc signaling is associated with insulin resistance, we evaluated the role of O-GlcNAc transferase (OGT), the enzyme that mediates O-GlcNAcylation, in skeletal muscle.

Global Trade Item Number

SKUGTIN
ABS19404053252633966